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Volume 5, Issue 1, 2000, pp. 1-5

Regioselective alkylation at the N4 position of a 3-oxo-1,4-benzodiazepine on solid support

Fadia E. Ali
Department of Medicinal Chemistry, Research and Development Division, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, P.O. Box 1539, King of Prussia, PA 19406-0939, U.S.A.
To whom correspondence should be addressed.
E-mail: fadia_e_ali@sbphrd.com

Catherine C.K. Yuan
Department of Medicinal Chemistry, Research and Development Division, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, P.O. Box 1539, King of Prussia, PA 19406-0939, U.S.A.

Stephen T. Ross
Department of Medicinal Chemistry, Research and Development Division, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, P.O. Box 1539, King of Prussia, PA 19406-0939, U.S.A.

Leon B. Hall
Department of Medicinal Chemistry, Research and Development Division, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, P.O. Box 1539, King of Prussia, PA 19406-0939, U.S.A.

Abstract
An efficient solid phase regioselective alkylation at the N4 position of a 3-oxo-1,4-benzodiazepine template exemplified by 4-H-2,3,4,5-tetrahydro-7-iodo-3-oxo-1H-1,4-benzodiazepine-2-acetate-polymer ester is described. Further chemical elaboration at position 7, utilizing a modified Heck reaction, allows the incorporation of amides from primary or secondary amines. The two diversity points at positions 4 and 7 were utilized to synthesize a 28-membered, combinatorial array on Sasrin. resin in moderate yields and > 80% purity. Having validated the chemistry on solid support, a combine and split approach to prepare a bead-bound combinatorial library is achievable utilizing similar experimental practices and procedures as in the array synthesis.

Keywords
benzodiazepine, combinatorial array, diversity, Heck reaction, regioselective alkylation, Sasrin.

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