Table of Contents
Filtration is Dead!
New Technique for High Throughput Synthesis
Compas 768.2 (Spyder Instruments, Inc.)
Separation of Solid & Liquid Phase
Centrifuge with Multiple Slingshots Leonardo da Vinci, Cod. Atl. F.57r.-b, 1488 (?)
Separation of Solid and Liquid Phase Principle of tilted centrifugation
Separation of Two Immiscible Liquid Phases Principle of tilted centrifugation extraction or fluorous phase synthesis
Applies to Wells of All Shapes
Microtiterplate Situation
Volume Considerations
Rotor With Adjustable Tilt
Contamination Issue
Is There a Danger of Bead Transfer? NO!
Compounds Synthesized in Neighboring Wells
Crude Model and First Prototype
PPT Slide
Multichannel Delivery of Solvents/Reagents
Compas 768.2
96 Channel Liquid Distributor
Detail of the Rotor
Internal or External Computer
Performing Individual Steps
Running Program
Setting Parameters
PPT Slide
Synthetic Center
Generating Compounds From Building Blocks
Editing Plate Layout
Protocol Editing
Program Creation
Plate Tracking
Program Tracking
Synthesis Algorithm
Mixing by Oscillation
Synthesis Algorithm (continuation)
HF Towers (Parallel cleavage in gas HF)
Result …
Or …
Or …
Or …
Is it Useful Today?
Example of Parallel Synthesis
Purity Evaluation (LC-MS TIC data)
LC-MS Evaluation of All Products
HPLCs of 768 Compounds Synthesized in One Batch
HPLCs of 768 Compounds Synthesized in One Batch
HPLCs of 768 Compounds Synthesized in One Batch
HPLCs of 768 Compounds Synthesized in One Batch
HPLCs of 768 Compounds Synthesized in One Batch
HPLCs of 768 Compounds Synthesized in One Batch
Is it Useful Today?
How About Miniaturization ?
Liquid Distribution
Liquid Removal
One Bead Per Well
Separation of Solid and Liquid Phase Principle of tilted centrifugation
Parallel Liquid-Liquid Extraction
Complete Removal of Liquid from Array of Wells Principle of tilted centrifugation plate washing
How About Tomorrow ? When and where can I get one of these instruments?
Spyder Instruments
Family of COMPAS Instruments Centrifugation-Based Techniques
Conclusions
Acknowledgement
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